2X HRM OLC Mix

2X HRM OLC Mix is designed for High-resolution melting curve (HRM) analysis after Real-Time polymerase chain reaction.  Double strand of amplified DNA sequences separated when the temperature increases. This separation provides a very specific graph according to length and content of amplicons. Using high-precision intercalation dyes, the reduction in fluorescence level due to separation of DNA double strand is determined by instant readings of qPCR, thus different sequences can be discriminated. DNA Polymerase in the mix possesses the 5′->3′ DNA polymerase activity, 3′->5′ (proofreading) exonuclease activity and temperature-depended strand-displacement activity and generates blunt ends in the amplification products. Intended use is identification of sequence differences present in common loci in different organisms by amplification of target gene regions in nucleic acid isolates and high resolution melting curve (HRM) analysis.

Ordering

Cat. NoSizePrice
BS-Amp-106-500
500 RxnInquire
BS-Amp-106-1000
1000 RxnInquire
BS-Amp-106-5000
5000 RxnInquire

*For more information on pricing please contact us

Documents

Manual (TR)
Manual (EN)
MSDS

Overview

2X HRM OLC Mix is designed for High-resolution melting curve (HRM) analysis after Real-Time polymerase chain reaction.  Double strand of amplified DNA sequences separated when the temperature increases. This separation provides a very specific graph according to length and content of amplicons. Using high-precision intercalation dyes, the reduction in fluorescence level due to separation of DNA double strand is determined by instant readings of qPCR, thus different sequences can be discriminated. DNA Polymerase in the mix possesses the 5′->3′ DNA polymerase activity, 3′->5′ (proofreading) exonuclease activity and temperature-depended strand-displacement activity and generates blunt ends in the amplification products.

Identification of sequence differences present in common loci in different organisms by amplification of target gene regions in nucleic acid isolates and high resolution melting curve (HRM) analysis.