2X qPCR HRM Fusion Mix

2X qPCR HRM Fusion Mix contains intercalated dye and is suitable for post-amplification melting curve analysis. High resolution melting curve (HRM) analysis after real-time polymerase chain reaction. The opening of the double strand during temperature increase with respect to the sequence content and length of amplified amplicons provides a very specific graph. Using high-precision intercalation dyes, the reduction in the amount of fluorescence produced by opening the double strand is determined by instant readings and different sequences can be separated. The DNA Polymerase in the mix has 5 ‘-> 3’ DNA polymerase activity, 3 ‘-> 5’ (prodreareading) exonuclease activity, temperature-dependent strand-translocation activity and blunt ends in amplification products. Intended use is identification of sequence differences present in common loci in different organisms by amplification of target gene regions in nucleic acid isolates and high resolution melting curve (HRM) analysis.

Ordering

Cat. NoSizePrice
BS-AMP-103-500500 µLInquire
BS-AMP-103-10001000 µLInquire
BS-AMP-103-50005 mLInquire

*For more information on pricing please contact us

Documents

Manual (TR)
Manual (EN)
MSDS

Overview

Kit contains intercalated dye and is suitable for post-amplification melting curve analysis. High resolution melting curve (HRM) analysis after real-time polymerase chain reaction. The opening of the double strand during temperature increase with respect to the sequence content and length of amplified amplicons provides a very specific graph. Using high-precision intercalation dyes, the reduction in the amount of fluorescence produced by opening the double strand is determined by instant readings and different sequences can be separated. The DNA Polymerase in the mix has 5 ‘-> 3’ DNA polymerase activity, 3 ‘-> 5’ (prodreareading) exonuclease activity, temperature-dependent strand-translocation activity and blunt ends in amplification products.

Identification of sequence differences present in common loci in different organisms by amplification of target gene regions in nucleic acid isolates and high resolution melting curve (HRM) analysis.