Biospeedy® 6X DNA Loading Dye is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. The loading dye increases the density of the sample and they add colour to the sample, thereby simplifying the loading process. The solution contains dyes that, in a electric field, move toward the anode at predictable rates. It contains two different dyes (bromophenol blue and xylene cyanol FF) for visual tracking of DNA migration during electrophoresis. In 1% agarose gels, bromophenol blue migrates with 300 bp linear double-stranded DNA fragment, whereas xylene cyanol FF migrates at approximately the same rate as linear double-stranded DNA 4 kb length.
The loading dye suits well for the DNA samples dissolved either in water or in EDTA-containing buffer (as TE buffer). If DNA markers are not prediluted with the Loading dye solution, then mix: The loading buffer is 6x concentrated, that means you have to use it 5:1. For DNA markers, apply 0.1 µg per 1 mm of agarose gel lane width.
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DNA agarose gel analysis after DNA restriction digestions, ligation, or dephosphorylation
For use with agarose and non-denaturing polyacrilamide gels
Proven performance for DNA Ladders preparation
Optimized to protect samples from nuclease degradation, provide high density and for sample confinement in the well without diffusing out from it