Nucleic Acid Extraction Kits

Bio-Speedy® nucleic acid extraction products are optimized to provide maximum yield, purity and integrity from all types of samples. You can effectively isolate DNA, RNA and plasmids from all kinds of samples with our kit specific to your type of sample we produce with the Bio-Speedy® brand.

DNA Extraction Kits               

Intended Use: Bio-Speedy® Fast Microbial DNA Isolation Kit is used to obtain the genetic material quickly and practically, required for molecular detection of microorganisms from enriched samples, preenriched food, water, etc.

Principle: It is based on the isolation of the genetic material by the help of heat treatment and centrifugation from samples in food, water etc. containing microorganisms generated in pre-enrichment medium in accordance with the standard methods (ISO 6579, ISO 11290, ISO 16654 and so on) or isolated in solid and / or liquid medium for the purpose of species detection.

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Intended UseBio-Speedy® Becterial DNA Isolation Kit From Water Samples is used to obtain the genetic material required for molecular detection of bacteria found in water samples.

PrincipleThe kit is based on the principle that the genetic material in microorganism cells isolated from water samples by pretreatment such as “membrane filtration” or “centrifugation” is extracted from the cell by heat treatment and purified by a series of processes such as centrifugation, washing, filtration. Prior to application of the Bio-Speedy® Bacterial DNA Isolation Kit from Water Samples, the polycarbonate membrane having a pore size of 0.45 μm and a diameter width of 25 mm is used to provide to retain the bacterial cells in the membrane surface by filtration, or the water samples are centrifuged at high speed to precipitate microorganisms. It is recommended that the Bio-Speedy® Bacterial DNA Isolation Kit from Water Samples and the “Bio-Speedy® Dead Cell Elimination Kit” are used together in case when only living cells need to be detected from the bacteria present in the water samples. Thus, it is possible to eliminate the dead microorganisms in the water sample with the help of the enzyme and to detect only the remaining living microorganism cells.

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Intended UseIt is used to obtain bacterial DNA required for molecular analysis to be applied to isolated bacterial cultures.

PrincipleIt is based on the principle that the genetic material in bacterial cells is extracted by heat treatment and enzymatic process and isolated by a series of processes such as centrifugation, washing, filtration.

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Intended Use: It is used to molecular identification of animal tissues such as muscle, fat, gelatin, etc. in food samples, and used to obtain genetic material required for molecular analysis of meat-type determination analyzes.

Principle: It is based on the principle that the genetic material in animal tissues in foods containing meat and meat products is extracted by physical and chemical disruption, heat treatment and enzymatic reaction and isolated by a series of processes such as centrifugation, washing, filtration.

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Intended Use: It is used to obtain the genetic material required for the molecular detection of molds and yeast (fungi) in food samples.

Principle: It is based on the principle that the genetic material in molds and yeast cells in foods is extracted by physical separation, heat treatment and enzymatic removal from the cell and isolated by a series of processes such as centrifugation, washing, filtration.
It is recommended that it is used in conjunction with the “Bio-Speedy® Dead Cell Elimination Kit” in case only when living fungi in food sample need to be detected. At this point, it is possible to eliminate the dead microorganisms in food samples with the aid of enzymes and to detect only the remaining living mold and yeast cells.
The kit can be applied directly to homogenized food samples or to food samples that have been pre-enriched in liquid media.

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Intended Use: The kit is used to obtain the genetic material required for the molecular detection of plant cells in different matrices such as processed and unprocessed food, feed, seed specimens. Using the genetic material obtained as a result of the kit application, GMO (Genetically Modified Organism), food allergen etc. Element / plant specific molecular analyzes can be performed.

Principle: It is based on the principle of extraction of plant specific genetic material in different samples such as processed and unprocessed food, feed, seed specimens by physical and chemical fragmentation, heat treatment and enzymatic reaction and isolatation by series of processes such as centrifugation, washing, filtration.

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Intended Use: Obtaining the genetic material required for molecular detection of bacteria in food samples.

Principle: It is based on the principle that extraction of genetic material in the bacterial cells in food samples by heat treatment and enzymatic reaction and isolation by a series of processes such as centrifugation, washing and filtration.
It is recommended to use with the “Bio-Speedy® Dead Cell Elimination Kit” in case only when the live bacteria need to be detected from the food samples. At this point, it is possible to eliminate the dead bacteria in food samples with the aid of enzymes and to detect only the remaining living bacterial cells.
The kit can be applied either directly to homogenized food samples or to food samples that have been subjected to pre-enrichment in liquid media.

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Intended Use: The Bio-Speedy® Bacterial DNA Isolation Kit is used to obtain the bacterial DNA required for molecular analysis to be applied to isolated bacterial cultures.

Principle: It is based on the principle that the genetic material in bacterial cells is taken out of the cell by heat treatment and isolated by a series of processes such as centrifugation, washing, filtration (Sambrook et al., 2001). With the obtained genetic material, species identification of bacteria, specific gene search, etc. molecular analyzes are made.

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Intended Use: It is used to obtain fungal DNA which is required for molecular analysis to be applied to isolated fungi cultures.

Principle: It is based on the principle that the genetic material in mold and yeast (fungi) cells is extracted by heat treatment and enzymatic digestion and isolated by a series of processes such as centrifugation, washing, filtration.

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Intended Use: Kit is used for the isolation of bacterial genomic DNA from clinical specimens such as serum, cerebrospinal fluid (CSF), etc. or microbial isolates.

Principle: The kit is based on the principle that the genetic material in gram negative and positive bacterial cells is taken out of the cell by applying physical and biochemical processes and purified by spin column technique. Kit protocol is suitable for CDC (Centers for Disease Control and Prevention) guidelines.

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Intended Use: It is used to obtain the DNA required for molecular analyzes to be applied to plant samples.

Principle: It is based on the principle that the genetic material in various plant samples is extracted by heat treatment and enzymatic digestion and isolated by a series of processes such as centrifugation, washing, filtration.

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Intended Use: It is used to obtain the microbial DNA required for molecular analyzes to be applied to environmental samples such as soil, mud, etc.

Principle: It is based on the principle that microbial genetic material in environmental samples such as soil, mud, etc. is extracted by heat treatment and enzymatic extraction and isolated by a series of processes such as centrifugation, washing, filtration.

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Intended Use: : It is used to obtain the animal DNA required for molecular analyzes to be applied to textile samples such as cashmere, wool, etc.

Principle: : It is based on the principle that the genetic content (nucleic acids) contained in the animal material (animal hair) from which the textile products are obtained is extreacted by heat treatment and with various reagents and isolated by a series of processes such as centrifugation, washing, filtration.

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RNA Extraction Kits              

Intended Use: It is used to obtain the microbial RNA required for molecular analyzes to be applied to environmental samples such as Soil, mud, etc.

Principle: It is based on the removal of the microbial genetic material in the sample such as soil, mud, etc. by heat treatment, chemical and physical disruption methods and isolation of the RNA by a series of applications such as enzymatic treatment, centrifugation, washing, filtration.

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Intended Use: The Bio-Speedy® TriEasy Plant Total RNA Isolation Kit has been developed to rapidly and efficiently isolate RNA molecules of high quality and purity from different plant species and different plant tissues.

Principle: After the mechanical and chemical breakdown of plant cells, it is based on the precipitation of RNA with organic extraction followed by phase separation with alcohol. Can handle high amounts at the same time.

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Intended Use: The Kit is designed to provide total RNA isolation from biological fluids such as whole blood, serum and plasma.

Principle: It is a method designed for total RNA isolation from biological fluids such as whole blood, serum, plasma and various tissue samples by using optimized solutions for phase separation.

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Intended Use: The Kit is designed to provide RNA isolation from animal tissue and biological fluids such as whole blood, serum and plasma.

Principle: It is a method designed for the isolation of RNA from biological fluids such as whole blood, serum, plasma and various tissue samples by using special spin columns and optimized solutions.

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Intended Use: It is used to obtain bacterial RNA which is required for molecular analysis such as RT-PCR to be applied to isolated bacterial cultures.

Principle: It is based on the principle that the genetic material in bacterial cells is extracted by heat treatment, chemical and physical disintegration methods, and RNA is isolated by a series of applications such as enzymatic treatment, centrifugation, washing, filtration.

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Intended Use: It is used to obtain the bacterial RNA required for molecular analysis to be applied to isolated bacterial cultures.

Principle: It is based on the principle that the genetic material in bacterial cells is extracted mechanically and chemically and RNA is isolated by phase separation with organic extraction followed by precipitation with alcohol. Using this kit, high purity total RNA can be obtained from bacterial isolates practically and economically.

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Intended Use: It is used to obtain fungal RNA which is required for molecular analysis such as RT-PCR to be applied to isolated fungi cultures.

Principle: It is based on the principle that the genetic material in mold and yeast (fungi) cells is taken out of the cell by heat treatment, chemical and physical disruption methods, and the RNA is isolated by a series of applications such as enzymatic treatment, centrifugation, washing, filtration.

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NA Extraction Kits               

Intended Use: The kit is used for bacterial and viral nucleic acid isolation from blood and tissue specimens. It is suitable for use in molecular applications because of high weight nucleic acid (NA) yield.

Principle: It is based on the principle that the genetic material in animal tissues is extracted from the cell by physical and chemical disruption, heat treatment and enzymatic reaction and isolated by washing processes after attaching NA to the silica column in the presence of chaotropic salt.

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Intended Use: The kit is used for bacterial and viral nucleic acid isolation from animal stool samples. It is suitable to use in Molecular biology applications due to high quaility NA elution.

Principle: It is based on the principle that the genetic material in animal stool specimens is extracted by physical and chemical digestion, heat treatment and enzymatic reaction and isolated by inhibitor elimination, washing processes and purification of the nucleic acid by binding silica column in the presence of chaotropic salt.

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Intended Use: Kit is used for viral nucleic acid isolation from blood serum, blood plasma, Buffy Coat / white blood cells, mammalian cell culture, cerebrospinal fluid (CSF), respiratory tract etc., liquid body aspirates, liquefied phlegm supernatant, or body aspirate supernatant.

Principle: The kit is based on the principle that genetic material in viral particles of any lipid membrane or protein shell is extracted by physical, enzymatic and biochemical processes and purified by spin column technique. Kit protocol is suitable for CDC (Centers for Disease Control and Prevention) guidelines.

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Intended Use: Kit is designed to make high quality, high amount and pure genomic NA isolation from whole blood samples manually.

Principle: It is a fast and easy method for high-purity chromosomal NA isolation from full blood samples by using specific spin columns and optimized solutions which are specific to genomic NA.

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Intended Use: Kit is designed to obtain Genomic DNA suitable for Next Generation Sequence Analysis and microarray techniques from advanced molecular technologies manually in high quality, quantity and purity from whole blood and various tissue samples.

Principle: It is a method designed for chromosomal DNA isolation in high purity and high concentrations from whole blood and various tissue samples by using specific spin columns and optimized solutions which are specific to genomic DNA.

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Robotic NA Extraction Kits               

Intended Use: The kit is used to isolate the host genomic DNA, microbial DNA of bacteria and of parasites, etc. and viral nucleic acid (NA) from animal stool samples by using the RINA ™ M14 automatically.

Principle: Briefly; the user first obtains feces lysate liquefied and cleaned by homogenization, lysis and performing PCR inhibitor elimination outside of the robot. Then nucleic acid purification of this lysate is carried out in the RINA-M14 ™ robot. The details of the working principle are as follows: the feces (IC can be added if desired by the user) are homogenized with a vortex or homogenizer in the presence of detergent and PCR inhibitor-eliminating agent (FL Solution). During homogenization, the feces and microorganisms are completely or partially lysed depending on their type. Next, if a homogenizer is used, to get rid of the homogenization consumables in the tube such as the bead etc. the homogenate is centrifuged at 3,000 g for 1 second, and supernatant is transfered into a new tube. The physical lysis process is then continued by heat treatment. Subsequently the enzymatic (Proteinase K) lysis of the homogenate / lysate is completed. The feces is now lysate. The lysate is cleaned by centrifugation. Both NA’s and IC if added are binding to silica coated iron oxide microparticles (Magnetic Bead Solution = MB Solution) in the presence of concentrated chaotropic salt, detergent mixture (LB Solution (IC can be added if requested by the user)), and alcohol (Solution B). Then the ICs and NAs bound to the MBs are cleaned and dried with the wash solution series (W1, W2 and W3) containing reduced chaotropic salt and increased alcohol, and then they are rinsed with the MBR Solution. After this step the robotic pipette tips are cleaned once again with TR Solution (the first one was at the stage of desiccation). Subsequently, the solution is washed with E solution containing salt at low concentration, and the purification is completed by passing the ICs and NAs through the MB solution to the E solution. Finally, if DNA sequencing (Sanger, New Generation, etc.) is to be performed with the obtained NA it should be considered whether or not there is IC in the eluate.

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Intended Use: The kit is used to isolate the host genomic DNA, microbial DNA of bacteria and of parasites, etc. and viral nucleic acid (NA) from animal tissue samples and blood samples by using the RINA ™ M14 automatically.

Principle: Briefly; the user first obtains tissue lysate liquefied and cleaned by homogenization, lysis and performing PCR inhibitor elimination outside of the robot. Then nucleic acid purification of this lysate is carried out in the RINA-M14 ™ robot. The details of the working principle are as follows: the feces (IC can be added if desired by the user) are homogenized with a vortex or homogenizer in the presence of detergent and PCR inhibitor-eliminating agent (TL Solution). During homogenization, the tissue and microorganisms are completely or partially lysed depending on their type. Next, if a homogenizer is used, to get rid of the homogenization consumables in the tube such as the bead etc. the homogenate is centrifuged at 3,000 g for 1 second, and supernatant is transfered into a new tube. The physical lysis process is then continued by heat treatment. Subsequently the enzymatic (Proteinase K) lysis of the homogenate / lysate is completed. The feces is now lysate. The lysate is cleaned by centrifugation. Both NA’s and IC if added are binding to silica coated iron oxide microparticles (Magnetic Bead Solution = MB Solution) in the presence of concentrated chaotropic salt, detergent mixture (LB Solution (IC can be added if requested by the user)), and alcohol (Solution B). Then the ICs and NAs bound to the MBs are cleaned and dried with the wash solution series (W1, W2 and W3) containing reduced chaotropic salt and increased alcohol, and then they are rinsed with the MBR Solution. After this step the robotic pipette tips are cleaned once again with TR Solution (the first one was at the stage of desiccation). Subsequently, the solution is washed with E solution containing salt at low concentration, and the purification is completed by passing the ICs and NAs through the MB solution to the E solution. Finally, if DNA sequencing (Sanger, New Generation, etc.) is to be performed with the obtained NA it should be considered whether or not there is IC in the eluate.

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Intended Use: The Kit; is used for viral nucleic acid (VNA) isolation from nasal swabs, nasopharyngeal swabs, nasopharyngeal aspirate, bronchoalveolar lavage, sputum and other respiratory system samples (such as mucus-containing specimens to prevent pipetting, physical beating with glass beads, or incubation with dithiothreitol / n-acetyl-cysteine treatment is needed), mammalian sera, mammalian cell cultures and their media by using RINA ™ M14 automatically.

Principle: First, with the presence of Proteinase K enzyme, with the presence of a concentrated chaotropic salt with CRNA and / or IC which were added by user and with the presence of detergent mixture (Lysis Solution), any viral particles with lipid membrane or protein sheath (or viral or cell cultures containing IC) is broken down and VNA occurs. Also both IC and VNA are bound to silica coated iron oxide nanoparticles (magnetic beads = MB) in the presence of the chaotropic salt on which alcohol is added. Subsequently, with wash solution series (WB1, WB2, and WB3) in which the chaotropic salt reduced and alcohol is increased ICCs and VNAs associated with MBs are cleaned and dried. Robotic pipette tips are cleaned with Wash Solution after this step. Then, by washing with EB containing salt at low concentration, ICs and VNAs are passed through MB to the EB and the purification is completed. The CRNA acts as a crowding agent and increases the total amount of NA in the environment together with possible low-level target VNAs, which is necessary for NA purification. Because the purification of NA’s are very inefficient under a certain concentration. It also indirectly protects VNAs some of which are as targets for nuclease in the potential nuclease presence. IC is used to check if the VNA purification yield and subsequent Real Time PCR reaction inhibition are within certain limits. For example, PhCine Distemper Virus (PDV) when used in combination with the appropriate Bio-Speedy® Respiratory System Viral Factor Real Time PCR Panel such as “Bio-Speedy® Influenzae, Influenza B, Human RNazP Targeted Multiple 2X RT- qPCR Mixture” the result should be positive. A negative result may indicate a low in purification yield or an inhibition in the Real Time PCR reaction. It should not be forgotten that if DNA sequencing (Sanger, New Generation etc.) is done with the final VNA, the results will be affected by the presence of CRNA and IC in the eluate.

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