Real-Time PCR

Real-Time PCR can be used for both qualitative and quantitative analysis; choosing the best method for your application requires a broad knowledge of this technology. Bio-Speedy® Real-Time PCR kits with high sensitive results provide Real-Time PCR services tailored to a variety of template and target species.

Intended Use: Amplification of target gene regions in nucleic acid isolates and real-time monitoring with hydrolysis probes used.

Highlights: it is suitable to work with various DNA templates at high efficiency with the optimized mixture. Compatible with all real-time PCR devices. It produces reproducible results with the stabilizers it contains against inhibitors. Due to its Hot-start feature, it does not give the primer dimer and does not lose activity at room temperature. Provides short activation time and improved PCR precision.

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Intended Use: The amplification of target gene regions in nucleic acid isolates and the determination of the differences in amplicons by melting curve analysis without the need for agarose electrophoresis.

Highlights: it is suitable to work with various DNA templates at high efficiency with the optimized mixture. Compatible with all real-time PCR devices. It produces reproducible results with the stabilizers it contains against inhibitors. Due to its Hot-start feature, it does not give the primer dimer and does not lose activity at room temperature. Provides short activation time and improved PCR precision.

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Laboratories, there is growing demand for reliable assays that are simple, convenient, and can be readily adapted to run in a high-throughput fashion. Bioeksen R & D Technologies offers assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization.

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Biospeedy®qPCR Strips and Plates Products include thin-wall PCR tubes, PCR plates, and accessories, all of which are precisely manufactured for optimal fit and performance with all kits.

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Intended Use: Amplification of target gene regions in nucleic acid isolates and real-time monitoring with the hydrolysis probes.

Highlights: Bio-Speedy® Colorless 2X One-Step RT-qPCR Mix offers the most advanced inhibitor and stabilizer chemistry combinations. The combined reverse transcriptase and hot-start DNA polymerase system provides fast, high-specific-specific and ultra-sensitive single-step RT-PCR analysis in a single step. It can work with not only RNA but also DNA templates in one step. Due to its Hot-start feature, it does not give the primer dimer and does not lose activity at room temperature. Provides short activation time and improved PCR precision. It is designed and optimized for hydrolysis probes.

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Intended Use: Amplification of target gene regions in nucleic acid isolates, real-time monitoring and melting curve analysis in one step.

Highlights: Bio-Speedy® Green 2X One-Step RT-qPCR Mix offers the most advanced inhibitor and stabilizer chemistry combinations. The combined reverse transcriptase and hot-start DNA polymerase system provides fast, high-specific-specific and ultra-sensitive single-step RT-PCR analysis in a single step. It can work with not only RNA but also DNA templates in one step. Due to its Hot-start feature, it does not give the primer dimer and does not lose activity at room temperature. Provides short activation time and improved PCR precision.

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Intended Use: Bio-Speedy® Taq DNA Polymerase produced for use in general PCR applications, multiplex PCR, gene mutation studies, T / A cloning applications.

Highlights: Bio-Speedy® Taq DNA Polymerase provides high product yield. It is a cost-effective product compared to competitors.

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Intended Use: Bio-Speedy® Fusion DNA Polymerase offers great advantages over challenging reactions, rapid amplifications and Long PCR experiments. It is designed for High fidelity PCR (Hi-Fi PCR), cloning and suitable to use direct blood sample as template.

Highlights: It has special design and 3 ‘→ 5’ exonuclease activity which enables to work at high accuracy. It gives accurate results up to 50 fold compared to standard polymerase enzymes. It has temperature-dependent strand-displacement activity.

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Intended Use: Bio-Speedy® Hot Taq DNA Polymerase is produced to amplify complex genomic cDNA samples in Hot start, real-time PCR and multiplex PCR reactions.

Highlights: Bio-Speedy® Hot Taq DNA Polymerase is optimized to stay inactive at lower temperatures, hence it does not loses its activity at room temperature. It reduces  primer dimer formation. Provides short activation time and improved PCR precision. It is a cost effective product compared to competitors.

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Intended Use: Amplification of target gene regions in nucleic acid isolates.

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Intended Use: CDNA synthesis in RNA isolates and amplification of target gene regions.

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Intended Use: Bio-Speedy® Reverse Transcriptase is suitable for applications such as RT-PCR, cDNA synthesis, primer extension analysis, mRNA 5′-end mapping, DNA-end labeling and dideoxynucleotide sequencing.

Highlights: Its produced by using an RNA dependent DNA polymerase gene of Moloney Murine Leukemia virüs. Bio-Speedy® Reverse Transcriptase offers High yield product. It is a cost effective product compared to competititors.

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