Robotic Nucleic Acid Extraction Kits

Bio-Speedy® robotic nucleic acid isolation kits are optimized to provide maximum yield, purity and integrity from all types of samples. You can effectively isolate DNA, RNA and plasmids from all kinds of samples with our kit specific to your type of sample we produce with the Bio-Speedy® brand.

Intended Use: The kit is used to isolate the host genomic DNA, microbial DNA of bacteria and of parasites, etc. and viral nucleic acid (NA) from animal stool samples by using the RINA ™ M14 automatically.

Principle: Briefly; the user first obtains feces lysate liquefied and cleaned by homogenization, lysis and performing PCR inhibitor elimination outside of the robot. Then nucleic acid purification of this lysate is carried out in the RINA-M14 ™ robot. The details of the working principle are as follows: the feces (IC can be added if desired by the user) are homogenized with a vortex or homogenizer in the presence of detergent and PCR inhibitor-eliminating agent (FL Solution). During homogenization, the feces and microorganisms are completely or partially lysed depending on their type. Next, if a homogenizer is used, to get rid of the homogenization consumables in the tube such as the bead etc. the homogenate is centrifuged at 3,000 g for 1 second, and supernatant is transfered into a new tube. The physical lysis process is then continued by heat treatment. Subsequently the enzymatic (Proteinase K) lysis of the homogenate / lysate is completed. The feces is now lysate. The lysate is cleaned by centrifugation. Both NA’s and IC if added are binding to silica coated iron oxide microparticles (Magnetic Bead Solution = MB Solution) in the presence of concentrated chaotropic salt, detergent mixture (LB Solution (IC can be added if requested by the user)), and alcohol (Solution B). Then the ICs and NAs bound to the MBs are cleaned and dried with the wash solution series (W1, W2 and W3) containing reduced chaotropic salt and increased alcohol, and then they are rinsed with the MBR Solution. After this step the robotic pipette tips are cleaned once again with TR Solution (the first one was at the stage of desiccation). Subsequently, the solution is washed with E solution containing salt at low concentration, and the purification is completed by passing the ICs and NAs through the MB solution to the E solution. Finally, if DNA sequencing (Sanger, New Generation, etc.) is to be performed with the obtained NA it should be considered whether or not there is IC in the eluate.

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Intended Use: The kit is used to isolate the host genomic DNA, microbial DNA of bacteria and of parasites, etc. and viral nucleic acid (NA) from animal tissue samples and blood samples by using the RINA ™ M14 automatically.

Principle: Briefly; the user first obtains tissue lysate liquefied and cleaned by homogenization, lysis and performing PCR inhibitor elimination outside of the robot. Then nucleic acid purification of this lysate is carried out in the RINA-M14 ™ robot. The details of the working principle are as follows: the feces (IC can be added if desired by the user) are homogenized with a vortex or homogenizer in the presence of detergent and PCR inhibitor-eliminating agent (TL Solution). During homogenization, the tissue and microorganisms are completely or partially lysed depending on their type. Next, if a homogenizer is used, to get rid of the homogenization consumables in the tube such as the bead etc. the homogenate is centrifuged at 3,000 g for 1 second, and supernatant is transfered into a new tube. The physical lysis process is then continued by heat treatment. Subsequently the enzymatic (Proteinase K) lysis of the homogenate / lysate is completed. The feces is now lysate. The lysate is cleaned by centrifugation. Both NA’s and IC if added are binding to silica coated iron oxide microparticles (Magnetic Bead Solution = MB Solution) in the presence of concentrated chaotropic salt, detergent mixture (LB Solution (IC can be added if requested by the user)), and alcohol (Solution B). Then the ICs and NAs bound to the MBs are cleaned and dried with the wash solution series (W1, W2 and W3) containing reduced chaotropic salt and increased alcohol, and then they are rinsed with the MBR Solution. After this step the robotic pipette tips are cleaned once again with TR Solution (the first one was at the stage of desiccation). Subsequently, the solution is washed with E solution containing salt at low concentration, and the purification is completed by passing the ICs and NAs through the MB solution to the E solution. Finally, if DNA sequencing (Sanger, New Generation, etc.) is to be performed with the obtained NA it should be considered whether or not there is IC in the eluate.

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Intended Use: The Kit; is used for viral nucleic acid (VNA) isolation from nasal swabs, nasopharyngeal swabs, nasopharyngeal aspirate, bronchoalveolar lavage, sputum and other respiratory system samples (such as mucus-containing specimens to prevent pipetting, physical beating with glass beads, or incubation with dithiothreitol / n-acetyl-cysteine treatment is needed), mammalian sera, mammalian cell cultures and their media by using RINA ™ M14 automatically.

Principle: First, with the presence of Proteinase K enzyme, with the presence of a concentrated chaotropic salt with CRNA and / or IC which were added by user and with the presence of detergent mixture (Lysis Solution), any viral particles with lipid membrane or protein sheath (or viral or cell cultures containing IC) is broken down and VNA occurs. Also both IC and VNA are bound to silica coated iron oxide nanoparticles (magnetic beads = MB) in the presence of the chaotropic salt on which alcohol is added. Subsequently, with wash solution series (WB1, WB2, and WB3) in which the chaotropic salt reduced and alcohol is increased ICCs and VNAs associated with MBs are cleaned and dried. Robotic pipette tips are cleaned with Wash Solution after this step. Then, by washing with EB containing salt at low concentration, ICs and VNAs are passed through MB to the EB and the purification is completed. The CRNA acts as a crowding agent and increases the total amount of NA in the environment together with possible low-level target VNAs, which is necessary for NA purification. Because the purification of NA’s are very inefficient under a certain concentration. It also indirectly protects VNAs some of which are as targets for nuclease in the potential nuclease presence. IC is used to check if the VNA purification yield and subsequent Real Time PCR reaction inhibition are within certain limits. For example, PhCine Distemper Virus (PDV) when used in combination with the appropriate Bio-Speedy® Respiratory System Viral Factor Real Time PCR Panel such as “Bio-Speedy® Influenzae, Influenza B, Human RNazP Targeted Multiple 2X RT- qPCR Mixture” the result should be positive. A negative result may indicate a low in purification yield or an inhibition in the Real Time PCR reaction. It should not be forgotten that if DNA sequencing (Sanger, New Generation etc.) is done with the final VNA, the results will be affected by the presence of CRNA and IC in the eluate.

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